Martin Luther University Halle-Wittenberg

ACS Pharmacology

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Hinderberger Group - Instruments

electron paramagnetic resonance devices

W-Band
Bruker/Cryogenics

Our high filed pulsed EPR spectrometer running at W-band regime (3.4T/ 94GHz). The W-band uses a Bruker E680 console, a TeraFlex microwave bridge along with an oxford IPS-120 power supply. A cryogen free magnet (Cryogenics 2017) with an integrated VTI (Virtual Temperature insert) produces magnetic fields up to 6T and enables operations between 2K and room temperature. Magnet operation is controlled by Cryogenic software and as of the Bruker console, we use Xepr. The setup is equipped with an ENDOR cylindrical resonator (EN600-1021H, Bruker).

X-Band
Bruker

The Elexsys E580 Bruker spectrometer, operating at X-band range (0.3T /9.7GHz) at both cw and pulse modes. We use a closed cycle cryostat (ARS- 4WH, www.arscryo.com) to supply Helium. We got a digital upgrade of the system (Bruker Biospin, 2019) on microwave bridge controller, Hall field controller, signal processing unit (SPU), SpecJet-III (0.5 ns of transient resoloution) and PatternJet-II Pulse Programmer.

A second MW source (Magnettech GmbH) is provided for electron double resonance experiments. The X-band frequency is amplified either by a 1kW Traveling Wave Tube (TWT-Applied system Engineering, USA) or a 300W-solid state amplifier (Bruker Biospin, 2022). An arbitrary waveform generator (SpinJet AWG, Bruker, sampling rate of 1.6 GS/s and 0.625 ns time resolution) enables us to use shaped pulses (Bruker Biospin, 2022).

The X-Band is also equipped with an RF power amplifier (ENI 3200L, 200W, 250KHz-150MHz), DICE pulsed ENDOR unit (E560D-P-RF).

e setup has two flexline split-ring resonators ER4118X–MS3 (pulsed EPR) and a flexline probehead ER4118X–MD4 for ENDOR measurements.

Q-Band

Q-Band

Q-Band
Bruker

The EMX-plus Q- spectrometer from Bruker, operating at Q-band range (1.2T /34GHz)in the CW-mode. We use a closed cycle croyostat (ARS- 4WH) along with a SOMITOMO-F70 compressor and Mercury iTC (Oxford Instruments) to reach and control temperature ranges between 5 and 300K. Qunitina is equipped with an ER5106QT/W cylindrical probehead.

Miniscope 5000

Miniscope 5000

MS 5000 mit Multi-Wavelength Fiber Coupled LEDs

MS 5000 mit Multi-Wavelength Fiber Coupled LEDs

Multi-Wavelength Fiber Coupled LEDs

Multi-Wavelength Fiber Coupled LEDs

MS5000
Freiberg Instruments/Bruker

The benchtop electron spin resonance (EPR) spectrometer measures  paramagnetic species at X-Band frequencies in continuous-wave (CW) mode.  Measurements of solid or liquid samples with a volume of 12-100 µL can  be performed at temperatures from the boiling point of liquid nitrogen  up to 200 °C. EPR spectroscopy is suitable for the investigation of  (bio)chemical systems with strongly localized spin density and their  interaction with the environment. It provides information about  structure and dynamics of the system of interest.

Multi-Wavelength Fiber Coupled LEDs
Prizmatix

The fiber-coupled high power UV, Blue, Green, Red and NIR LED multi  wavelength light source modules are effective replacements of lasers  and lamps in many applications. We use them for photochemical reactions  and irradiation of samples inside the MS5000 EPR spectrometer to  investigate photochemical radical formation or decay.

Miniscop 400

Miniscop 400

Miniscope MS400
Magnetech

Both spectrometers are used for research (spin probing, spin trapping and spin counting), as well as training. These spectrometers can be operated in a temperature range from -180°C up to 200°C. They are equipped with rectangular cavity TE102. Maximum available field range is up to 6500G. Their sensitivity is about 108-109 spins/G. Nitrogen temperature measurements are possible using liquid nitrogen Dewar.

optical devices

Litesizer Anton Paar

Litesizer Anton Paar

Litesizer
Anton Paar

The Litesizer 500 is used to characterize particles in dispersions. Static light scattering at 90° determines the molecular weight, dynamic light scattering at 15°, 90° and 175° is used to calculate the particle size, and with electrophoretic light scattering (cmPALS) the zeta potential is investigated. Furthermore, the transmissions and refractive indices of solutions can be measured.

Fluoromax-2

Fluoromax-2

Fluoromax-2
ISA/ Horiba

Monolith NT.115r

Monolith NT.115r

Monolith NT.115r
NanoTemper

With the Monolith NT 115r from NanoTemper, the method of microscale thermophoresis can be used to analyze the binding between molecules, e.g. enzymes and ligands, through the measurement of fluorescence intensity differences. For that, capillaries with a varying amount of target molecule and a constant amount of green or blue fluorescent binding partner get radiated with an IR-Laser to generate a temperature gradient. Because of the “Soret-Effekt”, bound and unbound molecules diffuse away from the “warm” detection region with different velocities, resulting in a different decrease of the fluorescence intensity, depending on the ratio of bound to unbound molecules. A graphic plot of the normed intensity against the varying concentration of one binding partner enables the fit of a binding curve and the precise determination of dissociation constants (KD-values) in the micro- to nanomolar range.

film Balance

Compression Film Balance

Compression Film Balance

Compression Film Balance

In a Teflon trough with moveable barriers, the surface pressure at the water-air interface is measured by a Wilhelmy plate. A surface-active  substance, like phospholipids dissolved in chloroform or polymers can  be spread unto an aqueous subphase. Chloroform is left to evaporate and  the molecules to equilibrate before compressing the molecules with the  barriers. Thereby the available molecular surface area is reduced  leading to an increase in surface pressure depending on the phase of the  molecule. An area isotherm is measured giving information about phase  transitions of the molecules in a monolayer at the water-air interface.

Adsorption Film Balance

Adsorption Film Balance

Adsorption Film Balance

A Wilhemly plate measures the surface pressure at the water-air  interface. Phospholipids or other surface active molecules can be  spread unto an aqueous subphase in a Teflon trough. Using a syringe,  molecules or proteins can be injected into this subphase directly  through an injection hole in the trough or by injection through or unto  the surface from above. This allows measurement of the surface pressure  increase and gives information of for example of protein lipid-monolayer interaction.

Fluoreszenzspektroscope

Fluoreszenzspektroscope

Fluoreszenzspektroscope

other devices

Refractometer

Refractometer

Refractometer
Anton Paar

The Anton Paar Abbemat 450 heavy-duty refractometer enables refractive index and concentration measurements to be made with an accuracy of ± 0.0001 nD. Due to the particularly robust design, aggressive chemicals can also be measured. The measurable temperature range is from 5-125° C. Due to the small sample well, only 200 µl sample volume is required for a measurement. The device can be controlled both via a digital control unit and via a computer.

Reometer AntonPaar

Reometer AntonPaar

Reometer
Anton Paar

The Rheometer Physika MCR 301 from Anton Paar is equipped with different measuring systems. We have Coneplate (CP) and PlatePlate (PP) measuring systems of various sizes, so samples with small amounts of approx. 180-200 µl or sample volumes of up to 2 ml can be measured. The rheometer enables the measurement of viscosity and viscoelastic behavior of liquid or solid samples.

qNano IZON

qNano IZON

TRPS (qNano)
IZON

Tunable Resistive Pulse Sensing (TRPS) measures individual particles size, concentration and zeta potential with incredibly high precision and accuracy. TRPS is more accurate than commonly used light scattering techniques that provide bulk estimates. The qNano Gold can be used for Particles that exeed 30 nm. The concentration of particles in the fluid as a number of particles per unit volume of fluid, across a specified detectable particle size range. An accurate size distribution of these particles plotted as a histogram of concentration v particle diameter (or volume). TRPS is the technology that delivers these fundamental requirements, and in addition can measure the surface charge of individual nanoparticles.

Äkta Pure

Äkta Pure

Äkta Pure
Cytiva

VP-ITC

VP-ITC

VP-ITC
MicroCalorimeter

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